Gram-positive bacteria stain purple due to the thick peptidoglycan layer which is not easily penetrated by the decolorizer Gram-negative bacteria, with their thinner peptidoglycan layer and higher lipid content, destain with the decolorizer and are counterstained red when Safranin is added (Figure 3). In the final step, a counterstain, Safranin, is added to color cells red (Figure 2). The slide is flushed with an Iodine solution, which fixes the Crystal Violet to the cell wall, followed by a decolorizer (an alcohol) to wash away any non-fixed Crystal Violet. The heat-fixed sample is stained with Crystal Violet, turning the cells purple. Briefly, a bacterial smear is placed on a microscope slide and then heat-fixed to adhere the cells to the slide and make them more readily accepting of stains (1). The Gram stain, developed in 1884 by the Danish bacteriologist Hans Christian Gram (1), differentiates bacteria based on the composition of the cell wall (1, 2, 3, 4). The most important parts of the microscope are labeled. Here, we will discuss visualizing bacteria with Gram stains, Capsule stains, and Endospore stains.įigure 1: A typical compound microscope. Typically, compound microscopes have multiple objective lenses of varying powers to allow for different magnification (1, 2). Combining the objective lens and eyepiece allows for higher magnification than using a single lens alone. This is then magnified by the eyepiece (ocular lens) which enlarges the image. Compound microscopes have an objective lens close to the object which collects light to create an image of the object. Simple microscopes (for example a magnifying glass) have only one lens to magnify an object, while compound microscopes have several lenses to enhance magnification (Figure 1). The main difference between them is the number of lenses used to magnify the object. The two main types of light microscopes are simple and compound. Staining bacteria is necessary when distinguishing bacterial types with light microscopy. To observe these properties, one can use light microscopy however, some bacterial characteristics (for example size, lack of coloration, and refractive properties) make it hard to distinguish bacteria solely with a light microscope (1, 2). Many properties help distinguish them from each other, including but not limited to Gram-staining type, shape and arrangement, production of capsule, and formation of spores. DiRita 1ġ Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of Americaīacteria are diverse microorganisms found nearly everywhere on Earth.
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Your puppy will need to go out regularly to use the bathroom and for exercise. Ask your vet about a microchip for dogs as well. A name tag and collar is a must-have on every pet parent's puppy checklist to ensure your pup will find their way back to you in the event of a mishap. Of course, no one ever wants to think about their puppy getting lost, but the reality is that accidents happen, and sometimes, dogs get out. Provide as much information as you can, so if and when someone finds your dog, they know who to contact. On the identification tags, list your puppy's name, your phone number, your address, and maybe even your email address. You can try both and see which your pet prefers. There should be room to slip two fingers between the collar and your dog's neck to ensure there's enough space for your puppy to breathe comfortably. The collar should fit snugly so your pup can't slip out of it. You'll want your puppy to have a name tag attached to a collar in case your pooch gets loose. Talk to a vet about any new puppy issues, big or small. New puppy? Ask a vet anything - for free! With the right research, you will find the perfect one. This could be useful if you have a dog that has lengthy ears, such as a Cavalier King Charles Spaniel, or a breed with a flat face, such as a French bulldog. You can also look into breed-specific bowls. They help them slow down their eating, keep them mentally stimulated, and prevent boredom. If you have a pup who is a speed eater, puzzle bowls can be helpful. That being said, you might need to upgrade to a deeper dish as your puppy grows. This is because they are often smaller than their adult counterparts. First, shallow bowls are the best for puppies. There are a few things that you should keep in mind when it comes to selecting the right match for your pup. Keep in mind that some dogs are allergic to plastic, so you may want to opt for a stainless steel bowl. These are crucial so that your dog has the opportunity to eat and stay nourished and hydrated. The next item on your shopping list before you bring your new puppy home should be dog dishes/bowls.īe sure to remember to purchase two, as you will give your pet food and water out of these. Pro tip: It helps to line up a vet before you bring your dog home. Talk to a vet-they can tell you whether or not you've picked a good food for your pup. If you’re unsure about your food of choice’s quality, your best bet is to ask instead of guess. The same will go for when you eventually transition your pup away from puppy food and help them move on to adult food. Again, this should be done slowly and mindfully, as dogs have sensitive stomachs. If you find that your new puppy is not enjoying their food, you will have to transition them to a new one. In addition, you will also want to ensure that you’re keeping an eye on your puppy’s growth watch how your dog is growing instead of how much food they leave on the plate. There is plenty to learn when it comes to the topic of feeding your puppy, and one of the single most important facets of this is to ensure that you’re giving your dog food that is quality: more protein and less filler. This means that you will have to be cognizant of ingredients to include or skip when you’re searching for the perfect food for your pup. Unfortunately, not all dog food is equal. One thing to note here: When you first bring them home, it's recommended that you keep them on the same food that they were being fed previously, and then can slowly transition them to your puppy food preference. To set your pup up for long-term nutritional success, you must ensure that you selected the right puppy food for them. Puppies have unique feeding requirements. New puppy checklist: 10 essential items for your new dog 1. Ensuring that your new puppy has access to all of these items is crucial to creating a safe, healthy, and comfortable environment for them to grow in: time to start shopping. Whether this is your first furry family member or your fifth, making sure you have everything on this new puppy checklist is essential to helping your pup succeed in their new life. While bringing home a brand new puppy is loads of fun, it's also important that you have everything your new pet will need before they arrive at your house.īefore you take the plunge to be a pet parent, there are several questions you need to ask yourself: Are you ready to bring a new puppy into your home? Do you have all the essentials that you need to properly care for them? Have you thoroughly examined a checklist of must-have items before you committed to bringing your puppy home? Along with it, I would also mention the tendency of mouthpiece to have build up, and the small chamber. Things like the small battery do hinder the experience.
So he’s totally blown away when Ada Twist’s Aunt Bernice inherits an old house from ice-cream mogul Herbert Sherbert that is filled with countless rooms from all his favourite architectural periods. Ada Twist, Scientist, the companion picture book featuring the next kid from Iggy Peck's class, is available in September 2016. A hilarious, irreverent book about doing your own thing Meet Iggy Peck-creative, independent, and not afraid to express himself In the spirit of David Shannon’s No, David and Rosemary Wells’s Noisy Nora, Iggy Peck will delight readers looking for irreverent, inspired fun. Iggy Peck is an architect at his very core: When he’s not making houses out of food, his head is up in the clouds, dreaming of design. Also from the powerhouse author-illustrator team of Iggy Peck, Architect, is Rosie Revere, Engineer, a charming, witty picture book about believing in yourself and pursuing your passion. Iggy Peck loves to build things and solve problems, sometimes out of. He loves building too much to give it up! With Andrea Beaty's irresistible rhyming text and David Roberts's puckish illustrations, this book will charm creative kids everywhere, and amuse their sometimes bewildered parents. Written by Andrea Beatty and Illustrated by David Roberts. His parents are proud of his fabulous creations, though they're sometimes surprised by his materials-who could forget the tower he built of dirty diapers? When his second-grade teacher declares her dislike of architecture, Iggy faces a challenge. A hilarious, irreverent book about doing your own thing Meet Iggy Peck-creative, independent, and not afraid to express himself! In the spirit of David Shannon's No, David and Rosemary Wells's Noisy Nora, Iggy Peck will delight readers looking for irreverent, inspired fun. I've seen several Lockdown episodes of Animated (I'm still working my way through the show), and I can't say his personality was extremely memorable to me. That being said.I sort of feel the inverse about their personalities. And it's a shame, because there was some fantastic early artwork of him as a green Mad Max style muscle car. I don't think it's a bad design per-se, but it doesn't really stick with me. Yet another silver Transformer with a pretty unremarkable placement of car parts, not a lot of defining features (aside from the face-gun), and he turns into another modern supercar. I find the AOE design to be very bland and generic. I'm not even in-love with that particular design (I don't really care for the spikes, honestly), but I like that he has a memorable and distinct design. My TF Oneshots Masterlist and TF Series Masterlist can be found at the provided links.In terms of design, I much prefer Animated. If you’re interested in seeing my other works or making a commission, check out my pinned post. Hello, everyone! This is the masterlist for all of my Transformers headcanons. Oneshots oneshot tfa tf transformers transformers animated writing fanfiction request requests masterlist masterlists Busy Bee (Sentinel Prime x Bumblebee x Jazz) (NSFW).Synesthesia (Sentinel Prime x Bumblebee) (NSFW).Happiness Doesn’t Wait (Prowl x Bumblebee).Say It Again (Blurr x Ultra Magnus) (NSFW).Hatefuck (Ultra Magnus x Megatron) (NSFW).Movement (Optimus Prime x Megatron) (NSFW).From Eden (Optimus Prime x Megatron) (NSFW).Say My Name (Optimus Prime x Megatron) (NSFW).Through The Years (Optimus Prime x Megatron x Reader).Wasteland, Baby! (Optimus Prime x Megatron).Like Real People Do (Optimus Prime x Megatron).Skeletons, Skeletons (Optimus Prime x Megatron).To Build A Home (Optimus Prime x Megatron).Our Gentle Sin (Optimus Prime x Megatron).Dreaming Of You (Optimus Prime x Megatron).Blue Cosmos (Optimus Prime x Starscream).Evergreen Wasn’t Such A Bad Dream (Optimus Prime x Starscream).Orion’s Belt (Optimus Prime x Bumblebee) (NSFW).Telling (Optimus Prime x Bumblebee) (NSFW). A Sight To See (Optimus Prime x Bumblebee).Everything Left Unsaid (Optimus Prime x Sentinel Prime) (NSFW).History (Optimus Prime x Sentinel Prime).Put Your Head On My Shoulder (Optimus Prime x Sentinel Prime).Training Wheels (Optimus Prime x Sentinel Prime).We Don’t Make Sense (Optimus Prime x Sentinel Prime).Happier (Optimus Prime x Sentinel Prime).Sleeping Soundly (Ratchet x Optimus Prime) (NSFW).Quiet Resource (Ratchet x Optimus Prime).And The Flowers Were Grey (Ratchet x Optimus Prime).Any Other Way (Blackarachnia x Optimus Prime).A Running Stream (Slipstream x Reader) (NSFW).What’s Yours Is Mine And What’s Mine Is Mine (Starscream x Reader x Megatron) (NSFW).Trials And Tribulation, Smiles And Manipulation (Starscream x Reader x Megatron) (NSFW).What’s Yours Is Mine And What’s Mine Is Mine (Megatron x Reader x Starscream) (NSFW).Trials And Tribulation, Smiles And Manipulation (Megatron x Reader x Starscream) (NSFW).Play Stupid Games, Win Stupid Prizes (Megatron x Reader x Ultra Magnus) (NSFW).Through The Years (Megatron x Reader x Optimus Prime).Feel It In The Sunrise (Megatron x Reader).Play Stupid Games, Win Stupid Prizes (Ultra Magnus x Reader x Megatron) (NSFW).Give Me Time (Ultra Magnus x Reader) (NSFW).A Piece Of You (Ultra Magnus x Reader) (NSFW).Are You Bored Yet? (Ultra Magnus x Reader).At The Shrine Of Your Lies (Sentinel Prime x Reader) (NSFW).Poison Paradise (Sentinel Prime x Reader) (NSFW).Out In The Open (Sentinel Prime x Reader) (NSFW).White Linen, White Rose (Bulkhead x Reader).When Your Actions Have Consequences (Optimus Prime x Reader) (NSFW).
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Like any other activity on the internet, some risks are involved even if using VPN or Proxy servers, so make sure to read the privacy policies of all services used before you start playing and be mindful of not disclosing any personal information when taking part in online gaming activities. It is always best practice not to use your personal profile or email ID when logging in instead, create an anonymous account that won’t be linked back to you and make sure no friends who can link you with the account know anything about your online gaming activities. Once you are able to unblock the game, it is important to consider a few safety precautions while playing Doodle Jump. How to Play doodle jump unblocked At School or Work? These cloud gaming platforms let you stream and play full versions of games on your browser so you can easily access it from any school or work computer without having to install additional software. Cloud Gaming ServiceĪnother great way to access and play Doodle Jump unblocked is by using Cloud gaming services such as PlayStation Now or Google Stadia. This allows gamers to access the game from another computer or Chrome device this could be either your own laptop, friends’ laptops, etc. There are many free proxy websites as well as paid ones, so it is important to do some research before you opt for a particular service.Īnother way of unblocking games on computers is with Google Chrome Remote Desktop. Unlike VPNs, proxies don’t offer encryption and hence they can connect faster than VPNs. ProxyĪnother option to access games on school or work computers is using proxy servers. The app will encrypt the internet traffic to convert it into a secure tunnel and hence bypass all firewalls this way you can access Doodle Jump unblocked without anyone tracking your online activities such as your IP address & location. To do this, you would need to download and install a reliable VPN app on your computer or smartphone with any of the available platforms like PC, Mac, iOS, or Android. A VPN can help bypass firewalls for gaming and protect your data privacy when playing online games. One of the simplest and most secure ways to unblock games is by connecting to an encrypted Virtual Private Network (VPN). There are many benefits to creating a doodle jump extension, such as adding more content to the game and keeping people coming back for more.Is Doodle Jump unblocked available offline? How To Unblock Doodle Jump? VPN If you’re a fan of the game, then you might be interested in creating your own doodle jump extension. Introduction to doodle jump extensionsĭoodle jump extensions are a great way to add more content to the popular mobile game Doodle Jump. If you’re a fan of Doodle Jump, then you might be interested in creating your own doodle jump extension. It’s simple yet addicting, and there’s always something new to discover. Think about what would make your doodle jump extension unique and fun to play, and start designing it today!ĭoodle Jump is one of the most popular mobile games out there, and for good reason. Here are some ideas for what your doodle jump extension could include: This would be a great way to add more content to the game and keep people coming back for more. If you’re a fan of the popular mobile game Doodle Jump, then you might be interested in creating a doodle jump extension. Go to Doodle Jump Extension (Chrome Web Store).4.8/5 - (25 votes) How to install Doodle Jump Extension Toshiba B3/462LF1BMHD EQPIII 840 BRAKE MOTOR-0. Right click on Computer icon and select Manage. Fix Driver Problems Faster with our Driver Update Utility. Price protection, price matching or price guarantees do not apply to Intra-day, Daily Deals or limited-time promotions. 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Samsung 840 evo driver windows 7 install - Driver Download for your Windows System from a verified website. #Allen datagraph 840 windows 7 drivers installĬustomer may be required to run system self-test programs or correct reported faults by following advice given over phone. #Allen datagraph 840 windows 7 drivers driverįile Name: samsung-840-evo-driver-windows-7-install.exe Version: 1.3.7 Driver Date: 24 February 2019 File Size: 1,675 KB Rating: 4.75/5. 1) Choose Operation System: Downloaded: 94,751 times Last Time. On-site services provided only if issue can't be corrected remotely. Service not available holidays and weekends. HP will transfer your name and address information, IP address, products ordered and associated costs and other personal information related to processing your application to Bill Me Later®. Bill Me Later will use that data under its privacy policy. Microsoft Windows 10: Not all features are available in all editions or versions of Windows 10. Systems may require upgraded and/or separately purchased hardware, drivers, software or BIOS update to take full advantage of Windows 10 functionality. #Allen datagraph 840 windows 7 drivers update Windows 10 is automatically updated, which is always enabled. ISP fees may apply and additional requirements may apply over time for updates. #Allen datagraph 840 windows 7 drivers driver.#Allen datagraph 840 windows 7 drivers update.#Allen datagraph 840 windows 7 drivers drivers. #Allen datagraph 840 windows 7 drivers install. Serve the chops with all the delicious juices poured over and garnish with the sprigs of thyme.Īs this is very rich you need to keep the vegetables quite simple. Then bake in the oven for 1 hour exactly. Place a sprig of thyme on each, then wrap up loosely in the foil, sealing securely, but leaving some space between the chops and the foil. Now spoon the mixture over the pork chops, then, finally, top each one with a large blob of crème fraîche. Let the lemon juice bubble for about 1 minute, then sprinkle in the flour and stir with a wooden spoon, absolutely ignoring what it looks like as all will be well in the end. When the juices start to run, drain the porcini, squeeze out any excess moisture, then chop them small and add them to the fresh mushrooms along with the lemon juice. Now chop the open-cap mushrooms roughly and fry them in the same pan you browned the meat in, adding a little more butter if you think it needs it. As you brown them, transfer them on to the foil, then season each one with salt and freshly milled black pepper and sprinkle on the thyme. Now, in a large frying pan, melt the butter and fry the chops to a nice, nutty, golden colour on both sides. Then place a sheet of foil (large enough to wrap the chops in) in the roasting tin. It will last anywhere from 4 – 6 months.What should you be cooking in October? (45)įirst of all, place the porcini mushrooms in a bowl, pour on enough boiling water just to cover them, then leave them to soak for 20 minutes. To extend the shelf life, I recommend freezing it! Wrap well or transfer to an airtight container and store in the freezer. Both the pork chops and mushrooms sauce will last in the fridge for 3 days! Freezing Wrap leftovers tightly or transfer to an airtight container. Serving this pork with a side of rice or any vegetable, with a nice piece of crusty bread is also an excellent idea. The list of of sides you can pair this dish with is endless! I personally love this dish with mashed potatoes. Spoon sauce over chops and serve with cooked rice. Return pork chops to the skillet and heat just until warmed through. Whisk and cook over low heat, just until sauce thickens (about 2 minutes). Add mushrooms and flour, parsley, and milk to the skillet. Mushrooms absorb water like sponges, therefore they won’t brown nicely when cooked. In a separate bowl, toss mushrooms with flour. Using bone-in pork chops will provide more flavor than using boneless pork chops.However, I never wash mushrooms because they will get waterlogged! To clean them, use a damp paper towel or a soft mushroom brush to wipe each mushroom to remove any dirt. Mushrooms grow low to the ground so they will usually carry a bit of dirt when you buy them from the stores. Garnish with fresh parsley and serve with mashed potatoes. Step 2: Brown Pork Chops Brown the chops in hot oil until they’re cooked through, then remove to a plate.
He has participated in mission trips all over the world including most recently in Mexico and Nicaragua. Mali has given his time and contributed his medical expertise to worthy organizations such as Medical Ministry International, Georgia Lions Camp for the Blind, Columbia Lighthouse for the Blind, and Baha’i inspired Health for Humanity. He is the proud member of the Manatee County Medical Society (MCMS), Sarasota County Medical Society (SCMS), Florida Medical Association (FMA), and Florida Ophthalmological Society (FSO). In addition to these honors, he was also selected for membership in the Golden Key International Society, American Society of Retina Specialists (ASRS), American Society of Cataract and Refractive Surgeons (ASCRS), and the American Academy of Ophthalmology (AAO). He has also been recognized by several honored organizations including Alpha Epsilon Delta (serving as vice-president for the WVU chapter), a prestigious National Premedical Honor Society, Beta Beta Beta (WVU Chapter), a National Biological Honor Society, and the National Society of Collegiate Scholars. In addition, he was awarded the 2019 West Virginia University Homecoming Parade Marshal in honor of his successful career and service to his community. He was also recently honored with the 2018 Outstanding Alumnus for the West Virginia University Department of Biology for being a leader in his field and all his success as an accomplished retinal surgeon and innovator. The American Society of Retina Specialists recently bestowed upon him the new Fellow of the ASRS (FASRS) designation which is awarded based to the retinal specialist who has demonstrated consistent commitment to the Society, expertise in the management of vitreoretinal disorders, and the highest ethical and professional standards. He also was recently recognized by his peers with the 2022 Healio Honors award which is award to healthcare professional that go above and beyond in their fields for their patients. Additionally, he was recognized as the 2021 Castle Connolly Top Doctor. Mali was named 2020 America’s Top Ophthalmologist. In addition, his retinal surgical video was included in the American Society of Retinal Specialists (ASRS) Innovative Retina Surgical Video Series. He has presented at national and international research meetings and has given lectures across the country. Mali is a principal investigator for many clinical trials including a new treatment for wet macular degeneration, diabetic retinopathy, and uveitis. Mali has been featured as a medical expert in both TV and Films, most recently in the PBS Documentary Film: Medical Stories: Finding Light in the Darkness.ĭr. He also hosts a regular monthly video retina blog on the Ophthalmology 360 platform: The Retina Project. Additionally, he hosts a worldwide broadcasted award-winning Podcast: Eye Care Insider with Dr. He has published research in esteemed medical publications including JAMA Ophthalmology, is a regular contributing author for Ophthalmology Times and Healio, and serves on the editorial advisory board of Ocular Surgery News, Ophthalmology 360, Modern Retina, and HCPLive. Mali utilizes the most advanced surgical techniques and technology in the world to deliver the highest quality retinal surgery for his patients. Mali is the Founder & CEO of Mali Enterprises, a very successful ophthalmology product company.ĭr. Mali is a board-certified ophthalmologist and award-winning retinal specialist for The Eye Associates, a multispecialty ophthalmology private practice in Sarasota, Florida and is an Attending Physician at Manatee Memorial Hospital (Bradenton, FL) where he performs retinal surgeries. During his fellowship, he became one of the first retinal specialists in the United States to use Iluvien, a new steroid implant for diabetic macular edema.Ĭurrently, Dr. He also did his medical and surgical Vitreo-Retinal fellowship training at Retina Consultants/Albany Medical College while serving as an Assistant Professor of Ophthalmology. Upon deciding to specialize in ophthalmology, he went on to Albany Medical College for his residency and was elected Chief Resident by his peers and faculty. He then continued at WVU School of Medicine for his medical degree and internship in which he was awarded the WV Medical Scholarship Award. Joshua Mali graduated Summa Cum Laude in Biology from West Virginia University (WVU) where he received the prestigious WVU Foundation Outstanding Senior Award. I'm dedicated to preserving my patients' sight with the most advanced treatments available.ĭr. You can too.) It makes the cookies chewier, and also it sounds exotic. **Glucose? The pastry chef at Momofoku (in NY city- super-cool place I honestly have only read about and have yet to go to) uses this in her amazing cookies, so there.In a separate bowl, beat butter, sugars and vanilla together until creamy. You cannot cream melted oil with sugar, so don’t even try it my friend.) How To Make This Recipe Combine the flour, baking soda and salt in a bowl. (Microwave if coconut oil is too stiff if oil melts, place in freezer until firm again like softened butter. *Vegan? Replace butter with coconut oil, softened to the consistency of soft butter.Allow cookies to cool on baking sheet for about 5 minutes before transferring to cooling racks. Place cookie dough balls on parchment-lined baking sheets and bake for 8-10 minutes, until slightly golden brown on tops. Preheat oven to 350° while cookies are chilling.Theyre a cross between a brownie and a sugar cookie wrapped in nuts. Using a fork, press down on cookies to flatten. Using a small or medium cookie scoop, scoop and drop cookie dough balls onto the prepared baking sheet, spaced evenly apart. The delicious cashew cookies wrapped in chopped cashews are a perfect snack. Using a rubber spatula, stir and fold until thoroughly mixed. Scoop heaping tablespoon-sized amounts of dough onto parchment-lined cookie sheets, cover with plastic wrap and refrigerate for at least three hours (up to three days). Enjoy the crunchy bite of cashew paired with yummy chocolate in every bite.Slowly add the dry ingredients to the wet ingredients until fully combined. In a separate bowl, beat together the butter, sugars and vanilla until light and fluffy. Stir in white chocolate and cashews until evenly distributed. In a medium bowl, combine the flours and baking soda. Add to wet mixture and mix until just combined, about 1 minute. In a medium bowl, whisk flour, baking soda, and salt together.In a large bowl, combine butter, egg, sugars, vanilla and glucose (if using), and beat on medium-high speed for 3-5 minutes until light and fluffy.That’s positive enough indeed.ġ tablespoon glucose** (totally optional)ġ cup (6 ounces) white chocolate chips/ chunks I must end on a positive note, not an early-senility one. 8-ounce (by weight) package brick-style cream cheese, softened (I used Philadelphia in the silver package) 1 cup (2 sticks, 8 ounces, pound) salted butter. Is that normal? Do I need to join Luminosity again?) My point is that I blanked on the fact that I had made a dessert with the two key ingredients in the first place. (Sidebar: I have to admit that I am slightly losing my mind… I searched my own site when linking the cookie recipe above, and realized that I had already married white chocolate and cashews together- in the White Chocolate Cashew Blondie like a year and a half ago. (Really- macadamia nuts will set you back a pretty penny. It’s totally compatible with white chocolate, and it has that richly satisfying texture that macadamia nuts have to offer- but for a fraction of the absolutely insane price that macadamias are known for. (And we all love it, I know, especially when you add a hint of lime to the situation.)Īh, yes. Yeah, we’ve all heard about the White Chocolate Macadamia Nut Cookie. |
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